Abstract: The present work focused on the effect of pyrolysis temperatures on the yield of bio-oil from fast pyrolysis of straw in a fluidized bed reactor. The chemical compositions of biooil, obtained at 300℃~600℃ coupling with condensation at 22℃ or －4.4℃, were examined by GC/MS and FT-IR. The results show that the highest bio-oil yield of 43.1% is obtained at 400℃. The condensation temperature significantly affects the quality of bio-oil. Lower condensation temperature facilitates increasing of its heat value and the content of organics, and consequently decreasing the concentration of water. Based on the present result, the subsequent technology could be flexibly set up to meet the objective requirements and obtain operation parameters to optimize the pyrolysis process.