Abstract: In this paper, a series of TiO₂ supported vanadium pentoxide and copper oxide catalysts were prepared by the impregnation method for selective catalytic reduction of NO_x with NH₃ at middle-low temperature (200~300℃). The catalysts were characterized by BET and X-ray diffraction (XRD). The results showed that 2V16Cu/TiO₂ calcinated at 500℃ exhibited the best catalytic activity at middle-low temperature and quite wide temperature window. A 97.3% NO_x conversion was obtained at 225℃ and a gross hourly space velocity (GHSV) of 6.0×10⁴h^-1, and it reached 100% at 250~300℃. XRD results showed that the characteristic peak of CuO gradually emerged with the increase of CuO loading. CuO was the key active component of the VCu/TiO₂ catalysts, and affected the catalytic activity at middle-low temperature significantly. The BET results showed that the CuO loading had great influence on surface structure of vanadium-titanium catalyst. The test of sulfur resistance performance showed that 2V16Cu/TiO₂ which was prepared by the introduction of vanadium pentoxide improved its sulfur resistance performance slightly.