Abstract: Ni/Al₂O₃ catalyst was selected as the reference catalyst for steam reforming of model bio-oil to produce hydrogen. Ni-Cu/Al₂O₃, Ni-Co/Al₂O₃ and Ni-Co-Cu/Al₂O₃ were prepared to investigate the influence of Co and Ni on steam reforming of bio-oil. The results show that Co can enhance the water gas shift (WGS) reaction rate, and Cu can prevent the formation of coke. The reaction conditions for the steam reforming of bio-oil with the Ni-Co-Cu/Al₂O₃ catalyst were optimized as the follows: temperature of 900 ℃, water-oil ratio (the mass ratio of steam to oil) of 6 g/g and weight hourly space velocity (WHSV) of 1 h^-1. The carbon selectivity of 87.5%, hydrogen yield of 84.2% and potential hydrogen yield of 92.4% can be obtained at the optimum conditions.